Coding
phaAB

Part:BBa_K282000:Design

Designed by: Kevin Chien   Group: iGEM09_Duke   (2009-09-30)

phaAB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 116
    Illegal NgoMIV site found at 212
    Illegal NgoMIV site found at 220
    Illegal NgoMIV site found at 232
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 569
    Illegal NgoMIV site found at 710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1809


Design Notes

Constructed using CPEC method.

Source

See below.

References

Valentin H and Dennis D. Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose. Journal of Biotechnology 58 (1997) 33–38.

Kidwell J, Valentin H, and Dennis, D. Regulated Expression of the Alcaligenes eutrophus pha Biosynthesis Genes in Escherichia coli. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Apr. 1995, p. 1391–1398.